@article{5507d58ce8c44170999a2d262253971c,
title = "Cep128 associates with Odf2 to form the subdistal appendage of the centriole",
abstract = "The mother centriole in a cell has two appendages, the distal appendage (DA) and subdistal appendage (SDA), which have roles in generating cilia and organizing the cellular microtubular network, respectively. In the knockout (KO) cells of Odf2, the component of the DA and SDA, both appendages simultaneously disappear. However, the molecular mechanisms by which the DA and SDA form independently but close to each other downstream of Odf2 are unknown. Here, using super-resolution structured illumination microscopy (SR-SIM), we found that the signal for GFP-tagged Odf2 overlapped considerably with that of immunofluorescently labeled Cep128. We further found that Cep128 knockdown (KD) caused the dissociation of other SDA components from the centriole, including centriolin, Ndel1, ninein and Cep170, whereas Odf2 was still associated with the centriole. In contrast, the DA components remained associated with the centriole in Cep128 KD cells. Consistent with this observation, we identified Cep128 as an Odf2-interacting protein by immunoprecipitation. Taken with the finding that Cep128 deletion decreased the stability of centriolar microtubules, our results indicate that Cep128 associates with Odf2 in the hierarchical assembly of SDA components to elicit the microtubule-organizing function.",
keywords = "Odf2, centrosome, microtubules, subdistal appendage, super-resolution structured illumination microscopy",
author = "Hiroka Kashihara and Shuhei Chiba and Kanno, {Shin ichiro} and Koya Suzuki and Tomoki Yano and Sachiko Tsukita",
note = "Funding Information: We are grateful to Drs. K. Mizuno, T. Inoue, T. Kobayashi, A. Inoko, H. Goto, M. Inagaki and S. Hirotsune for providing antibodies and plasmids. We thank S. Kawabata, K. Tateishi and F. Takenaga for technical assistance and laboratory members for discussion. We are grateful to Dr. H. Tanaka for critical comments on the manuscript. We thank the SR‐SIM facility at the Center for Medical Research and Education, Osaka University. This work was supported by the JSPS KAKENHI Grant‐in‐Aid for Young Scientists A (15H05596) to S.C and the Joint Research Program of the Joint Usage/Research Center at the Institute of Development, Aging and Cancer, Tohoku University, to S.C., A. Y. and S.K. This work was also supported in part by Grant‐in‐Aid for Scientific Research (A) (Grant no. 24247037, 18H03999) to S.T., Core Research for Evolutional Science and Technology (CREST) (Grant no. 11581) from the Japan Science and Technology Agency (JST) to S.T. and Grant‐in‐Aid for a JSPS Research Fellow (18J12466) to H.K. Funding Information: We are grateful to Drs. K. Mizuno, T. Inoue, T. Kobayashi, A. Inoko, H. Goto, M. Inagaki and S. Hirotsune for providing antibodies and plasmids. We thank S. Kawabata, K. Tateishi and F. Takenaga for technical assistance and laboratory members for discussion. We are grateful to Dr. H. Tanaka for critical comments on the manuscript. We thank the SR-SIM facility at the Center for Medical Research and Education, Osaka University. This work was supported by the JSPS KAKENHI Grant-in-Aid for Young Scientists A (15H05596) to S.C and the Joint Research Program of the Joint Usage/Research Center at the Institute of Development, Aging and Cancer, Tohoku University, to S.C., A. Y. and S.K. This work was also supported in part by Grant-in-Aid for Scientific Research (A) (Grant no. 24247037, 18H03999) to S.T., Core Research for Evolutional Science and Technology (CREST) (Grant no. 11581) from the Japan Science and Technology Agency (JST) to S.T. and Grant-in-Aid for a JSPS Research Fellow (18J12466) to H.K. Funding Information: Joint Research Program of the Joint Usage/Research Center at the Institute of Development, Aging and Cancer, Tohoku University; Japan Society for the Promotion of Science, Grant/Award Number: 15H05596, 18H03999, 18J12466 and 24247037; Core Research for Evolutional Science and Technology, Grant/Award Number: 11581 Publisher Copyright: {\textcopyright} 2019 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd",
year = "2019",
month = mar,
doi = "10.1111/gtc.12668",
language = "English",
volume = "24",
pages = "231--243",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "3",
}