@article{6e07f7a360924ccabf3ff39ddcfed9af,
title = "Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27",
abstract = "We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ-orfC-orfD-argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK. Purified LysK protein has deacetylating activities for both N2-acetyllysine and N2-acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus.",
keywords = "Arginine biosynthesis, Lysine biosynthesis, Thermus thermophilus, argE",
author = "Junichi Miyazaki and Nobuyuki Kobashi and Tomoyuki Fujii and Makoto Nishiyama and Hisakazu Yamane",
note = "Funding Information: We thank Dr. H. Nishida (Institute of Molecular and Cellular Biosciences, The University of Tokyo) for his assistance in phylogenetic analysis. This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.",
year = "2002",
month = feb,
day = "13",
doi = "10.1016/S0014-5793(02)02290-1",
language = "English",
volume = "512",
pages = "269--274",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "1-3",
}