Cloning and expression of an Endo-1,6-β-D-glucanase gene (neg1) from neurospora crassa

Seiji Oyama; Youhei Yamagata; Keietsu Abe; Tasuku Nakajima

doi:10.1271/bbb.66.1378

Cloning and expression of an Endo-1,6-β-D-glucanase gene (neg1) from neurospora crassa

Seiji Oyama, Youhei Yamagata, Keietsu Abe, Tasuku Nakajima

農学研究科・農芸化学専攻

研究成果: Article › 査読

13 被引用数 (Scopus)

抄録

A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.

本文言語	English
ページ（範囲）	1378-1381
ページ数	4
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	66
号	6
DOI	https://doi.org/10.1271/bbb.66.1378
出版ステータス	Published - 2002 1月 1

ASJC Scopus subject areas

バイオテクノロジー
分析化学
生化学
応用微生物学とバイオテクノロジー
分子生物学
有機化学

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10.1271/bbb.66.1378

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AU - Nakajima, Tasuku

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N2 - A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.

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KW - Fungal β-1,6-glucanase

KW - Neurospora crassa

KW - Recombinant β-1,6-glucanase

KW - β-1,6-glucanase gene

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Cloning and expression of an Endo-1,6-β-D-glucanase gene (neg1) from neurospora crassa

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