Cloning, expression, and transglycosylation reaction of Paenibacillus sp. strain W-61 xylanase 1

Seiji Watanabe, Dung Nguyen Viet, Jun Kaneko, Yoshiyuki Kamio, Shigeki Yoshida

研究成果: ジャーナルへの寄稿学術論文査読

13 被引用数 (Scopus)

抄録

A xylanase gene, xyn1, which encodes Paenibacillus sp. strain W-61 xylanase 1 (Xyn1), was cloned in Escherichia coli. xyn1 encodes 211 amino acid residues, including 28 amino acid residues of a signal peptide. The deduced amino acid sequence of the mature Xyn1 showed 95.7%, 84.0%, and 83.7% identity to family 11 xylanases of Aeromonas caviae ME-1, Paenibacillus sp., and Bacillus stearothermophilus respectively. The physico-chemical properties of recombinant Xyn1 were very similar to those of intact Xyn1, except for the molecular mass. The pattern of xylooligosaccharides generated by rXyn1 was investigated by fluorophore-assisted carbohydrate electrophoresis (FACE). The degradation rate of xylohexaose by rXyn1 increased markedly as compared with that of xylopentaose. Xylohexaose had a single preferential point of cleavage by rXyn1. On the basis of the pattern of action of xylooligosaccharides, the number of subsites was estimated to be six. The catalytic site was located between the third and the fourth subsites from non-reducing end.

本文言語英語
ページ(範囲)951-958
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
72
4
DOI
出版ステータス出版済み - 2008

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