TY - JOUR
T1 - CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect
AU - Anada, Takahisa
AU - Okada, Naoko
AU - Minari, Jusaku
AU - Karinaga, Ryouji
AU - Mizu, Masami
AU - Koumoto, Kazuya
AU - Shinkai, Seiji
AU - Sakurai, Kazuo
N1 - Funding Information:
This work was supported by the Japan Science and Technology Corporation (SORST Program). We also thank Taito Co., Japan, for providing the native schizophyllan.
PY - 2006/3/1
Y1 - 2006/3/1
N2 - Zymosan, classified among β-(1→3)-d-glucans, is produced from the cell wall of yeast and well known to induce proinflammatory cytokines when ingested by immune cells. We found that zymosan forms a complex with immunostimulatory CpG DNA, where both zymosan and CpG DNA can induce cytokine secretion according to the different mechanisms (i.e., recognized by different receptors). The complex activated macrophages and induced cytokine secretion, more efficiently than separate administration of zymosan or CpG DNA. Microscopic observation showed that this increment of the cytokine secretion can be explained by the fact that zymosan and zymosan/CpG DNA complex are up-taken more than naked CpG DNA. Additionally, existence of two different immunostimulants in the same cells may enhance the immunoresponse. This report presents a new strategy to construct a delivering vehicle for CpG DNA and to enhance its activity with the 'cocktail effect' of the two immunostimulants.
AB - Zymosan, classified among β-(1→3)-d-glucans, is produced from the cell wall of yeast and well known to induce proinflammatory cytokines when ingested by immune cells. We found that zymosan forms a complex with immunostimulatory CpG DNA, where both zymosan and CpG DNA can induce cytokine secretion according to the different mechanisms (i.e., recognized by different receptors). The complex activated macrophages and induced cytokine secretion, more efficiently than separate administration of zymosan or CpG DNA. Microscopic observation showed that this increment of the cytokine secretion can be explained by the fact that zymosan and zymosan/CpG DNA complex are up-taken more than naked CpG DNA. Additionally, existence of two different immunostimulants in the same cells may enhance the immunoresponse. This report presents a new strategy to construct a delivering vehicle for CpG DNA and to enhance its activity with the 'cocktail effect' of the two immunostimulants.
KW - CpG DNA
KW - Delivery
KW - Polysaccharide-polynucleotide complex
KW - Zymosan
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U2 - 10.1016/j.bmcl.2005.11.070
DO - 10.1016/j.bmcl.2005.11.070
M3 - Article
C2 - 16343899
AN - SCOPUS:31344448063
SN - 0960-894X
VL - 16
SP - 1301
EP - 1304
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 5
ER -