TY - JOUR
T1 - Cytoprotective effects of grape seed extract on human gingival fibroblasts in relation to its antioxidant potential
AU - Katsuda, Yusuke
AU - Niwano, Yoshimi
AU - Nakashima, Takuji
AU - Mokudai, Takayuki
AU - Nakamura, Keisuke
AU - Oizumi, Satomi
AU - Kanno, Taro
AU - Kanetaka, Hiroyasu
AU - Egusa, Hiroshi
N1 - Publisher Copyright:
© 2015 Katsuda et al.
PY - 2015/8/10
Y1 - 2015/8/10
N2 - Cytoprotective effects of short-term treatment with grape seed extract (GSE) upon human gingival fibroblasts (hGFs) were evaluated in relation to its antioxidant properties and compared with those of a water-soluble analog of vitamin E: trolox (Tx). GSE and Tx showed comparable antioxidant potential in vitro against di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH; a stable radical), hydroxyl radical (·OH), singlet oxygen (1O2), and hydrogen peroxide (H2O2). Pretreatment or concomitant treatment with GSE for 1 min protected hGFs from oxidative stressors, including H2O2, acid-electrolyzed water (AEW), and 1O2, and attenuated the intracellular formation of reactive oxygen species induced by H2O2 and AEW. Tx also reduced the H2O2- and AEW-induced intracellular formation of reactive oxygen species, but showed no cytoprotective effects on hGFs exposed to H2O2, AEW, or 1O2. These results suggest that the cytoprotective effects of GSE are likely exerted independently of its antioxidant potential.
AB - Cytoprotective effects of short-term treatment with grape seed extract (GSE) upon human gingival fibroblasts (hGFs) were evaluated in relation to its antioxidant properties and compared with those of a water-soluble analog of vitamin E: trolox (Tx). GSE and Tx showed comparable antioxidant potential in vitro against di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH; a stable radical), hydroxyl radical (·OH), singlet oxygen (1O2), and hydrogen peroxide (H2O2). Pretreatment or concomitant treatment with GSE for 1 min protected hGFs from oxidative stressors, including H2O2, acid-electrolyzed water (AEW), and 1O2, and attenuated the intracellular formation of reactive oxygen species induced by H2O2 and AEW. Tx also reduced the H2O2- and AEW-induced intracellular formation of reactive oxygen species, but showed no cytoprotective effects on hGFs exposed to H2O2, AEW, or 1O2. These results suggest that the cytoprotective effects of GSE are likely exerted independently of its antioxidant potential.
UR - http://www.scopus.com/inward/record.url?scp=84942456315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942456315&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0134704
DO - 10.1371/journal.pone.0134704
M3 - Article
C2 - 26258747
AN - SCOPUS:84942456315
SN - 1932-6203
VL - 10
JO - PLoS One
JF - PLoS One
IS - 8
M1 - e0134704
ER -