TY - JOUR
T1 - Dental pulp-derived mesenchymal stem cells for modeling genetic disorders
AU - Masuda, Keiji
AU - Han, Xu
AU - Kato, Hiroki
AU - Sato, Hiroshi
AU - Zhang, Yu
AU - Sun, Xiao
AU - Hirofuji, Yuta
AU - Yamaza, Haruyoshi
AU - Yamada, Aya
AU - Fukumoto, Satoshi
N1 - Funding Information:
Funding: This work was supported by the Japan Society for the Promotion of Science [KAKENHI; grant numbers JP19K10387, JP19K10406, JP19K19272, and JP19K10386].
Funding Information:
This work was supported by the Japan Society for the Promotion of Science [KAKENHI; grant numbers JP19K10387, JP19K10406, JP19K19272, and JP19K10386]. We thank the members of the Department of Pediatric Dentistry and Special Needs Dentistry at Kyushu University Hospital for their valuable suggestions, technical support, and materials. We appreciate the technical assistance provided by the Research Support Center at the Research Center for Human Disease Modeling, Kyushu University Graduate School of Medical Sciences.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3/1
Y1 - 2021/3/1
N2 - A subpopulation of mesenchymal stem cells, developmentally derived from multipotent neural crest cells that form multiple facial tissues, resides within the dental pulp of human teeth. These stem cells show high proliferative capacity in vitro and are multipotent, including adipogenic, myogenic, osteogenic, chondrogenic, and neurogenic potential. Teeth containing viable cells are harvested via minimally invasive procedures, based on various clinical diagnoses, but then usually discarded as medical waste, indicating the relatively low ethical considerations to reuse these cells for medical applications. Previous studies have demonstrated that stem cells derived from healthy subjects are an excellent source for cell-based medicine, tissue regeneration, and bioengineering. Furthermore, stem cells donated by patients affected by genetic disorders can serve as in vitro models of disease-specific genetic variants, indicating additional applications of these stem cells with high plasticity. This review discusses the benefits, limitations, and perspectives of patient-derived dental pulp stem cells as alternatives that may complement other excellent, yet incomplete stem cell models, such as induced pluripotent stem cells, together with our recent data.
AB - A subpopulation of mesenchymal stem cells, developmentally derived from multipotent neural crest cells that form multiple facial tissues, resides within the dental pulp of human teeth. These stem cells show high proliferative capacity in vitro and are multipotent, including adipogenic, myogenic, osteogenic, chondrogenic, and neurogenic potential. Teeth containing viable cells are harvested via minimally invasive procedures, based on various clinical diagnoses, but then usually discarded as medical waste, indicating the relatively low ethical considerations to reuse these cells for medical applications. Previous studies have demonstrated that stem cells derived from healthy subjects are an excellent source for cell-based medicine, tissue regeneration, and bioengineering. Furthermore, stem cells donated by patients affected by genetic disorders can serve as in vitro models of disease-specific genetic variants, indicating additional applications of these stem cells with high plasticity. This review discusses the benefits, limitations, and perspectives of patient-derived dental pulp stem cells as alternatives that may complement other excellent, yet incomplete stem cell models, such as induced pluripotent stem cells, together with our recent data.
KW - Dental pulp stem cells
KW - Disease model
KW - Mesenchymal stem cells
KW - Stem cells from human exfoliated deciduous teeth
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U2 - 10.3390/ijms22052269
DO - 10.3390/ijms22052269
M3 - Review article
C2 - 33668763
AN - SCOPUS:85101267927
SN - 1422-0067
VL - 22
SP - 1
EP - 18
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 5
M1 - 2269
ER -
