Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura; Kiyotaka Nakagawa; Yuko Saito; Kenji Yamagishi; Masahiro Suzuki; Kohji Yamaki; Hiroshi Shinmoto; Teruo Miyazawa

doi:10.1021/jf0306901

Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura, Kiyotaka Nakagawa, Yuko Saito, Kenji Yamagishi, Masahiro Suzuki, Kohji Yamaki, Hiroshi Shinmoto, Teruo Miyazawa

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

92 被引用数 (Scopus)

抄録

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

本文言語	英語
ページ（範囲）	1415-1418
ページ数	4
ジャーナル	Journal of Agricultural and Food Chemistry
巻	52
号	6
DOI	https://doi.org/10.1021/jf0306901
出版ステータス	出版済み - 2004 3月 24

文献へのアクセス

10.1021/jf0306901

他のファイルとリンク

フィンガープリント

「Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

@article{83a4217d1af34f24a83eaca7fbdaf1b3,

title = "Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection",

abstract = "A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.",

keywords = "1-Deoxynojirimycin, Evaporative light scattering detector, Morus alba, Mulberry leaves",

author = "Toshiyuki Kimura and Kiyotaka Nakagawa and Yuko Saito and Kenji Yamagishi and Masahiro Suzuki and Kohji Yamaki and Hiroshi Shinmoto and Teruo Miyazawa",

year = "2004",

month = mar,

day = "24",

doi = "10.1021/jf0306901",

language = "English",

volume = "52",

pages = "1415--1418",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

AU - Kimura, Toshiyuki

AU - Nakagawa, Kiyotaka

AU - Saito, Yuko

AU - Yamagishi, Kenji

AU - Suzuki, Masahiro

AU - Yamaki, Kohji

AU - Shinmoto, Hiroshi

AU - Miyazawa, Teruo

PY - 2004/3/24

Y1 - 2004/3/24

N2 - A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

AB - A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

KW - 1-Deoxynojirimycin

KW - Evaporative light scattering detector

KW - Morus alba

KW - Mulberry leaves

UR - http://www.scopus.com/inward/record.url?scp=1642351057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642351057&partnerID=8YFLogxK

U2 - 10.1021/jf0306901

DO - 10.1021/jf0306901

M3 - Article

C2 - 15030188

AN - SCOPUS:1642351057

SN - 0021-8561

VL - 52

SP - 1415

EP - 1418

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 6

ER -