Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura, Kiyotaka Nakagawa, Yuko Saito, Kenji Yamagishi, Masahiro Suzuki, Kohji Yamaki, Hiroshi Shinmoto, Teruo Miyazawa

研究成果: ジャーナルへの寄稿学術論文査読

89 被引用数 (Scopus)

抄録

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

本文言語英語
ページ(範囲)1415-1418
ページ数4
ジャーナルJournal of Agricultural and Food Chemistry
52
6
DOI
出版ステータス出版済み - 2004 3月 24

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