Effect of agonal and postmortem factors on gene expression profile: Quality control in microarray analyses of postmortem human brain

Hiroaki Tomita, Marquis P. Vawter, David M. Walsh, Simon J. Evans, Prabhakara V. Choudary, Jun Li, Kevin M. Overman, Mary E. Atz, Richard M. Myers, Edward G. Jones, Stanley J. Watson, Huda Akil, William E. Bunney

研究成果: Review article査読

267 被引用数 (Scopus)

抄録

There are major concerns that specific agonal conditions, including coma and hypoxia, might affect ribonucleic acid (RNA) integrity in postmortem brain studies. We report that agonal factors significantly affect RNA integrity and have a major impact on gene expression profiles in microarrays. In contrast to agonal factors, gender, age, and postmortem factors have less effect on gene expression profiles. The Average Correlation Index is proposed as a method for evaluating RNA integrity on the basis of similarity of microarray profiles. Reducing the variance due to agonal factors is critical in investigating small but validated gene expression differences in messenger RNA levels between psychiatric patients and control subjects.

本文言語English
ページ(範囲)346-352
ページ数7
ジャーナルBiological Psychiatry
55
4
DOI
出版ステータスPublished - 2004 2月 15
外部発表はい

ASJC Scopus subject areas

  • 生物学的精神医学

フィンガープリント

「Effect of agonal and postmortem factors on gene expression profile: Quality control in microarray analyses of postmortem human brain」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル