TY - JOUR
T1 - Effect of sulfobutyl ether-β-cyclodextrin and propylene glycol alginate on the solubility of clozapine
AU - Furuishi, Takayuki
AU - Sekino, Kohei
AU - Gunji, Mihoko
AU - Fukuzawa, Kaori
AU - Nagase, Hiromasa
AU - Endo, Tomohiro
AU - Ueda, Haruhisa
AU - Yonemochi, Etsuo
N1 - Funding Information:
This work was supported in part by a grant from the Strategic Research Foundation Grant-aided Project for Private Universities; a matching fund subsidy from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) 2008–2012 (to all authors); and partly by a Grant-in-Aid for Young Scientists (B) 25870795 from MEXT 2013–2014 in Japan and Hoshi University Grant-in-Aid for Leading Research Project 2017 (to T.F.).
Publisher Copyright:
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2019/4/21
Y1 - 2019/4/21
N2 - Clozapine (CLZ) is an atypical antipsychotic medication used in the treatment of schizophrenia and is poorly soluble in water (0.05 mM). In this study, we have investigated the effect of β-cyclodextrin (CD) and its derivatives on the solubility of CLZ. The solubility of the CLZ was measured to generate a phase solubility diagram, and the interaction between CLZ and sulfobutyl ether-β-cyclodextrin (SBE-β-CD) in aqueous solution was observed by 1 H- and 2D rotating-frame Overhauser enhancement spectroscopy (ROESY)-NMR methods. Moreover, the synergistic effect of SBE-β-CD and water-soluble polymers, including polyvinylpyrrolidone, hydroxypropyl methylcellulose, carboxymethylcellulose sodium salt, polyvinyl alcohol, sodium alginate, and propylene glycol alginate (PGA), on the solubility of CLZ was investigated. The results show that the solubility of CLZ with 1 w/v% PGA was 7.6 mM, which was almost four times greater than that of CLZ without PGA in a 15 mM SBE-β-CD solution. In contrast, the solubility of CLZ with 1 w/v % PGA in an aqueous solution decreased by one-third relative to that of CLZ in a 15 mM SBE-β-CD solution. 2D ROESY-NMR indicated that a CLZ/SBE-β-CD/PGA ternary complex formed. It was found that the combination of PGA and SBE-β-CD enhanced the solubility of CLZ.
AB - Clozapine (CLZ) is an atypical antipsychotic medication used in the treatment of schizophrenia and is poorly soluble in water (0.05 mM). In this study, we have investigated the effect of β-cyclodextrin (CD) and its derivatives on the solubility of CLZ. The solubility of the CLZ was measured to generate a phase solubility diagram, and the interaction between CLZ and sulfobutyl ether-β-cyclodextrin (SBE-β-CD) in aqueous solution was observed by 1 H- and 2D rotating-frame Overhauser enhancement spectroscopy (ROESY)-NMR methods. Moreover, the synergistic effect of SBE-β-CD and water-soluble polymers, including polyvinylpyrrolidone, hydroxypropyl methylcellulose, carboxymethylcellulose sodium salt, polyvinyl alcohol, sodium alginate, and propylene glycol alginate (PGA), on the solubility of CLZ was investigated. The results show that the solubility of CLZ with 1 w/v% PGA was 7.6 mM, which was almost four times greater than that of CLZ without PGA in a 15 mM SBE-β-CD solution. In contrast, the solubility of CLZ with 1 w/v % PGA in an aqueous solution decreased by one-third relative to that of CLZ in a 15 mM SBE-β-CD solution. 2D ROESY-NMR indicated that a CLZ/SBE-β-CD/PGA ternary complex formed. It was found that the combination of PGA and SBE-β-CD enhanced the solubility of CLZ.
KW - Clozapine
KW - NMR spectrum
KW - cyclodextrins
KW - propylene glycol alginate
KW - solubility
KW - ternary complex
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U2 - 10.1080/10837450.2018.1514521
DO - 10.1080/10837450.2018.1514521
M3 - Article
C2 - 30126299
AN - SCOPUS:85053371853
SN - 1083-7450
VL - 24
SP - 479
EP - 486
JO - Pharmaceutical Development and Technology
JF - Pharmaceutical Development and Technology
IS - 4
ER -