Electrochemical imaging for single-cell analysis of cell adhesion using a collagen-coated large-scale integration (LSI)-based amperometric device

Hiroya Abe, Yusuke Kanno, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

研究成果: ジャーナルへの寄稿総説査読

13 被引用数 (Scopus)

抄録

We here report the electrochemical imaging of cell adhesion using a large-scale integration (LSI)-based amperometric device, called a Bio-LSI device. The device consists of 400 sensor electrodes arranged with a pitch of 250μm. The device surface was modified with collagen to assist in the culture of MCF-7 cells and promote their adhesion. The cells disturb the electrochemical reaction of redox mediators, allowing the electrochemical signal to be used to evaluate cell adhesion at the single-cell level. This approach was applied to a cell detachment test. The results show that the Bio-LSI device is a promising tool for single-cell analysis.

本文言語英語
ページ(範囲)364-367
ページ数4
ジャーナルElectrochemistry
84
5
DOI
出版ステータス出版済み - 2016 5月

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