Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device

Kuniaki Nagamine, Tomoyuki Yasukawa, Hitoshi Shiku, Masahiro Koide, Tomosato Itayama, Tomokazu Matsue

研究成果: 会議への寄与学会論文査読

抄録

We have fabricated a microfluidic device comprising a 100 pL scale analytical chamber for electrochemical measurement of cellular activities. The microfluidic main channel and the analytical chamber were fabricated using poly(dimethylsiloxane) (PDMS). The chamber contained two Pt microelectrodes served as an electrochemical working electrode and an electrophoretic electrode to manipulate cells into the chamber. Yeast cells were introduced to the chamber by the electrophoresis, and its β-galactosidase (βGal) activities were characterized by amperometry. p-Aminophenol (PAP), generated by the enzyme-catalyzed hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG), was detected electrochemically.

本文言語英語
ページ645-647
ページ数3
出版ステータス出版済み - 2006
イベント10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, 日本
継続期間: 2006 11月 52006 11月 9

会議

会議10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
国/地域日本
CityTokyo
Period06/11/506/11/9

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