TY - JOUR
T1 - Enzymatic photochemical sensing using luciferase-immobilized polymer nanoparticles covered with artificial cell membrane
AU - Konno, Tomohiro
AU - Ito, Tomomi
AU - Takai, Madoka
AU - Ishihara, Kazuhiko
N1 - Funding Information:
A part of this research was carried out at the Center for NanoBio Integration, The University of Tokyo. This study was partially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (T. I. 16650098) and the Asahi Glass Foundation (2004). We thank Dr. Junji Watanabe, Osaka University, for his helpful discussions. One of the authors (T. I.) was supported by a Grant from the 21st Century COE Program, ‘Human-Friendly Materials Based on Chemistry’ from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2006/12
Y1 - 2006/12
N2 - We prepared phospholipid polymer nanoparticles immobilized with luciferase, and the nanoparticles were applied as photochemical sensing nanoparticles. An amphiphilic water-soluble polymer having a phosphorylcholine group was used as an emulsifier and a surface modifier to prepare the nanoparticles. The polymer was composed of three kinds of monomer units, that is, 2-methacryloyloxyethyl phosphorylcholine (MPC) as a hydrophilic and bioinert unit, n-butyl methacrylate as a hydrophobic unit and p-nitrophenyl ester having methacrylate as an enzyme-immobilizing unit. The p-nitrophenyl ester groups to immobilize the proteins were located on the surface of the nanoparticles. Luciferase was immobilized by the reaction between the p-nitrophenyl ester groups and the amino group. The enzymatic reaction on the nanoparticles was followed using a microdialysis system with an optical fiber having a 800 μm diameter in the probe. The nanoparticles conjugated with luciferase reacted with ATP, luciferin and oxygen. It is concluded that the nanoparticles are a promising tool for a photochemical sensing microdiagnostic system.
AB - We prepared phospholipid polymer nanoparticles immobilized with luciferase, and the nanoparticles were applied as photochemical sensing nanoparticles. An amphiphilic water-soluble polymer having a phosphorylcholine group was used as an emulsifier and a surface modifier to prepare the nanoparticles. The polymer was composed of three kinds of monomer units, that is, 2-methacryloyloxyethyl phosphorylcholine (MPC) as a hydrophilic and bioinert unit, n-butyl methacrylate as a hydrophobic unit and p-nitrophenyl ester having methacrylate as an enzyme-immobilizing unit. The p-nitrophenyl ester groups to immobilize the proteins were located on the surface of the nanoparticles. Luciferase was immobilized by the reaction between the p-nitrophenyl ester groups and the amino group. The enzymatic reaction on the nanoparticles was followed using a microdialysis system with an optical fiber having a 800 μm diameter in the probe. The nanoparticles conjugated with luciferase reacted with ATP, luciferin and oxygen. It is concluded that the nanoparticles are a promising tool for a photochemical sensing microdiagnostic system.
KW - 2-Methacryloyloxyethyl phosphorylcholine polymer
KW - Chemical luminescence
KW - Enzymatic reaction
KW - Nanoparticles
KW - Photosensing system
UR - http://www.scopus.com/inward/record.url?scp=33845247322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845247322&partnerID=8YFLogxK
U2 - 10.1163/156856206778937235
DO - 10.1163/156856206778937235
M3 - Article
C2 - 17260507
AN - SCOPUS:33845247322
SN - 0920-5063
VL - 17
SP - 1347
EP - 1357
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
IS - 12
ER -