TY - JOUR
T1 - Evaluation of the anti-hyperglycemic effect and safety of microorganism 1-deoxynojirimycin
AU - Takasu, Soo
AU - Parida, Isabella Supardi
AU - Onose, Shinji
AU - Ito, Junya
AU - Ikeda, Ryoichi
AU - Yamagishi, Kenji
AU - Higuchi, Oki
AU - Tanaka, Fukuyo
AU - Kimura, Toshiyuki
AU - Miyazawa, Teruo
AU - Nakagawa, Kiyotaka
N1 - Funding Information:
This work was supported in part by KAKENHI (Grant Number 16K07750 to TK) of Japan Society for the Promotion of Science, Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Asahimatsu Foods Co., Ltd. provided support in the form of salaries for author RI, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Biodynamic Plant Institute Co., Ltd. provided support in the form of remuneration for author OH, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2018 Takasu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2018/6
Y1 - 2018/6
N2 - 1-Deoxynojirimycin (DNJ) is a potent a-glucosidase inhibitor and thus beneficial for prevention of diabetes. While we have succeeded in obtaining the culture supernatant extract (CSE) rich in DNJ from microorganism source, information regarding its anti-hyperglycemic effect and safety were still limited. Therefore, this study was aimed to evaluate the antihyperglycemic effect and safety of microorganism DNJ. Oral sucrose tolerance test was performed, and the result showed that CSE was able to significantly suppress the blood glucose elevation and suggested DNJ as the main active compound. To determine its safety, the absorption and excretion of microorganism DNJ were evaluated using 15N labeling method. Our findings investigated the recovery rate of 15N from DNJ reached 80% up to 48 hours after oral administration, suggesting its rapid excretion, suggesting the safety of DNJ. This study verified the functional properties and safety of DNJ from microorganisms, suggesting its potential use for functional purpose.
AB - 1-Deoxynojirimycin (DNJ) is a potent a-glucosidase inhibitor and thus beneficial for prevention of diabetes. While we have succeeded in obtaining the culture supernatant extract (CSE) rich in DNJ from microorganism source, information regarding its anti-hyperglycemic effect and safety were still limited. Therefore, this study was aimed to evaluate the antihyperglycemic effect and safety of microorganism DNJ. Oral sucrose tolerance test was performed, and the result showed that CSE was able to significantly suppress the blood glucose elevation and suggested DNJ as the main active compound. To determine its safety, the absorption and excretion of microorganism DNJ were evaluated using 15N labeling method. Our findings investigated the recovery rate of 15N from DNJ reached 80% up to 48 hours after oral administration, suggesting its rapid excretion, suggesting the safety of DNJ. This study verified the functional properties and safety of DNJ from microorganisms, suggesting its potential use for functional purpose.
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U2 - 10.1371/journal.pone.0199057
DO - 10.1371/journal.pone.0199057
M3 - Article
C2 - 29897983
AN - SCOPUS:85048966951
SN - 1932-6203
VL - 13
JO - PLoS One
JF - PLoS One
IS - 6
M1 - e0199057
ER -
