TY - JOUR
T1 - Expression of myostatin in neural cells of the olfactory system
AU - Iwasaki, Shunsuke
AU - Miyake, Masato
AU - Watanabe, Hitoshi
AU - Kitagawa, Eri
AU - Watanabe, Kouichi
AU - Ohwada, Shyuichi
AU - Kitazawa, Haruki
AU - Rose, Michael T.
AU - Aso, Hisashi
N1 - Funding Information:
Acknowledgments We acknowledge the late Prof. Takahiro Yamaguchi for helpful comments. This study was supported by a Grant-in-Aid for Scientific Research (A) (No. 17208024) from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and also a Research project for utilizing advanced technologies in agriculture, forestry, and fisheries (No. 1523) from the Ministry of Agriculture, Forestry and Fisheries, Japan.
PY - 2013/2
Y1 - 2013/2
N2 - Recent studies show that myostatin mRNA expression is found in some regions of the brain. However, the functional significance of this is currently unknown. We therefore investigated myostatin expression and function in the brain. In this study, we used immunohistochemistry, in situ hybridization, and RT-PCR analysis to reveal that myostatin is expressed in the mitral cells in the olfactory bulb (OB) and in neurons in the olfactory cortex (OC). Using 3D reconstruction, mitral cells positive for myostatin were positioned in the lateral and ventral regions of the OB. In contrast, myostatin-positive mitral cells were detected in mice at 2 weeks of age, but not on days 0 and 7 after birth. Activin receptor IIB, a myostatin receptor, was expressed in the OB, OC, hippocampus, and paraventricular thalamic nucleus. Moreover, c-Fos immunostaining in granule cells in the OB was augmented after intracerebroventricular injection of myostatin. These findings suggest that myostatin is localized in specific cells associated with the olfactory system of the brain and may act as a key inhibitor in cell and/or signal development of the olfactory system.
AB - Recent studies show that myostatin mRNA expression is found in some regions of the brain. However, the functional significance of this is currently unknown. We therefore investigated myostatin expression and function in the brain. In this study, we used immunohistochemistry, in situ hybridization, and RT-PCR analysis to reveal that myostatin is expressed in the mitral cells in the olfactory bulb (OB) and in neurons in the olfactory cortex (OC). Using 3D reconstruction, mitral cells positive for myostatin were positioned in the lateral and ventral regions of the OB. In contrast, myostatin-positive mitral cells were detected in mice at 2 weeks of age, but not on days 0 and 7 after birth. Activin receptor IIB, a myostatin receptor, was expressed in the OB, OC, hippocampus, and paraventricular thalamic nucleus. Moreover, c-Fos immunostaining in granule cells in the OB was augmented after intracerebroventricular injection of myostatin. These findings suggest that myostatin is localized in specific cells associated with the olfactory system of the brain and may act as a key inhibitor in cell and/or signal development of the olfactory system.
KW - Brain
KW - Intracerebroventricular injection
KW - Myostatin
KW - Neurogenesis
KW - Olfactory system
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U2 - 10.1007/s12035-012-8342-1
DO - 10.1007/s12035-012-8342-1
M3 - Review article
C2 - 22941030
AN - SCOPUS:84872489704
SN - 0893-7648
VL - 47
SP - 1
EP - 8
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 1
ER -