TY - JOUR
T1 - Expression of versican and ADAMTS during rat tooth eruption
AU - Sone, Shinya
AU - Nakamura, Megumi
AU - Maruya, Yuriko
AU - Takahashi, Ichiro
AU - Mizoguchi, Itaru
AU - Mayanagi, Hideaki
AU - Sasano, Yasuyuki
N1 - Funding Information:
We wish to thank Mr. Masami Eguchi and Mr. Yasuto Mikami, Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, for their excellent assistance in this study. This work was supported in part by grants-in-aid (15390550, 16500285, 16390609) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2005/5
Y1 - 2005/5
N2 - A disintegrin and metalloprotease with thrombospondin type 1 motifs (ADAMTS) is a family of extracellular proteases and implicated in cleaving proteoglycans, such as aggrecan, versican and brevican. No information is available about expression or localization of these ADAMTSs in teeth. Versican is a large chondroitin sulfate proteoglycan that is present in a variety of connective tissue including dental pulp, dentin, cementum and periodontal ligaments. The present study was designed to investigate expression of ADAMTSs and versican during rat tooth eruption. Rat maxillary first molars in weeks 1, 2, 3, 4 and 6 were examined. The mRNA expression of ADAMTS1, ADAMTS4, ADAMTS5 and versican was localized using in situ hybridization. ADAMTS1, ADAMTS4, ADAMTS5 and versican were expressed in dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes. The temporal and spatial expression pattern in these cellular phenotypes was comparable among ADAMTSs and versican. The present study suggests that dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes may be involved in both production and degradation of versican with secreting ADAMTS1, ADAMTS4 and ADAMTS5.
AB - A disintegrin and metalloprotease with thrombospondin type 1 motifs (ADAMTS) is a family of extracellular proteases and implicated in cleaving proteoglycans, such as aggrecan, versican and brevican. No information is available about expression or localization of these ADAMTSs in teeth. Versican is a large chondroitin sulfate proteoglycan that is present in a variety of connective tissue including dental pulp, dentin, cementum and periodontal ligaments. The present study was designed to investigate expression of ADAMTSs and versican during rat tooth eruption. Rat maxillary first molars in weeks 1, 2, 3, 4 and 6 were examined. The mRNA expression of ADAMTS1, ADAMTS4, ADAMTS5 and versican was localized using in situ hybridization. ADAMTS1, ADAMTS4, ADAMTS5 and versican were expressed in dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes. The temporal and spatial expression pattern in these cellular phenotypes was comparable among ADAMTSs and versican. The present study suggests that dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes may be involved in both production and degradation of versican with secreting ADAMTS1, ADAMTS4 and ADAMTS5.
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U2 - 10.1007/s10735-005-5534-2
DO - 10.1007/s10735-005-5534-2
M3 - Article
C2 - 16200461
AN - SCOPUS:26244448721
SN - 1567-2379
VL - 36
SP - 281
EP - 288
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
IS - 4
ER -