Fibroblasts culture on polyacrylamide hydrogels with functional gradient

It is well known that complex multicellular process of tissue development and regeneration is generally regulated by the spatial arrangement of matrix and signal molecules in a time- and concentration-dependent manner. Based on the natural morphogenic process, various cell scaffolds of three-dimension have been designed to mimic the natural extracellular microenvironments that enable cells to regulate tissue regeneration. In this study, a polyacrylamide hydrogel scaffold with a spatial gradient of functional group has been explored to investigate the adhesion and proliferation of fibroblasts on the scaffold.