In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent

Shigeo Matsuyama, Naoki Hamada, Keizo Ishii, Yuichiro Nozawa, Satoru Ohkura, Atsuki Terakawa, Yoshinobu Hatori, Kota Fujiki, Mitsuhiro Fujiwara, Sho Toyama

研究成果: ジャーナルへの寄稿学術論文査読

6 被引用数 (Scopus)

抄録

In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.

本文言語英語
ページ(範囲)123-126
ページ数4
ジャーナルNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
348
DOI
出版ステータス出版済み - 2015

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