TY - JOUR
T1 - Induction of adrenomedullin by hypoxia and cobalt chloride in human colorectal carcinoma cells
AU - Nakayama, Masaharu
AU - Takahashi, Kazuhiro
AU - Murakami, Osamu
AU - Shirato, Kunio
AU - Shibahara, Shigeki
N1 - Funding Information:
This study has been supported partly by a Grant-in-aid for Scien-ti®c Research (C) (No. 09670117) from the Ministry of Education, Science, Sports and Culture of Japan (to K. T.), a Grant-in-aid for the Brain Science Research from the Ministry of Health and Welfare of Japan (to K. T.), and by the Gonryou Medical Foundation (to K. T.).
PY - 1998/2/13
Y1 - 1998/2/13
N2 - Adrenomedullin (ADM) is a vasodilator peptide, first isolated from human pheochromocytoma. To explore the pathophysiological role of ADM in ischemic conditions, we investigated the effects of hypoxia on ADM production and ADM mRNA expression in a cultured human colorectal carcinoma cell line, DLD-1. Northern blot analysis and radioimmunoassay showed that hypoxia stimulated the accumulation of ADM mRNA in the DLD-1 cells and immunoreactive ADM (ir-ADM) in the cultured media. Exposure to hypoxia for 12 hours increased ADM mRNA levels about 6-fold and ir-ADM levels about 4-fold. Moreover, treatment of DLD-1 cells with cobalt chloride, which mimics hypoxic states, significantly increased ADM mRNA levels about 18-fold and ir-ADM levels about 4-fold. These results suggest that ADM plays an important role in the pathophysiology of ischemic states.
AB - Adrenomedullin (ADM) is a vasodilator peptide, first isolated from human pheochromocytoma. To explore the pathophysiological role of ADM in ischemic conditions, we investigated the effects of hypoxia on ADM production and ADM mRNA expression in a cultured human colorectal carcinoma cell line, DLD-1. Northern blot analysis and radioimmunoassay showed that hypoxia stimulated the accumulation of ADM mRNA in the DLD-1 cells and immunoreactive ADM (ir-ADM) in the cultured media. Exposure to hypoxia for 12 hours increased ADM mRNA levels about 6-fold and ir-ADM levels about 4-fold. Moreover, treatment of DLD-1 cells with cobalt chloride, which mimics hypoxic states, significantly increased ADM mRNA levels about 18-fold and ir-ADM levels about 4-fold. These results suggest that ADM plays an important role in the pathophysiology of ischemic states.
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U2 - 10.1006/bbrc.1998.8131
DO - 10.1006/bbrc.1998.8131
M3 - Article
C2 - 9480840
AN - SCOPUS:0032512583
SN - 0006-291X
VL - 243
SP - 514
EP - 517
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -