TY - JOUR
T1 - Label-free detection of DNA molecules moving in micro-fluidic channels by infrared absorption spectroscopy
AU - Miyoshi, Tomoyuki
AU - Ishibashi, Ken ichi
AU - Miyamoto, Koichiro
AU - Hirano-Iwata, Ayumi
AU - Kimura, Yasuo
AU - Niwano, Michio
N1 - Funding Information:
This research has been carried out at the Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University. This work was partly supported by JST CREST .
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe solution-phase biomolecular species through IR spectral profiles of those biomolecules, allowing us to detect and separate biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transport of single-stranded DNA molecules (oligonucleotides) in the micro-fluidic channels.
AB - Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe solution-phase biomolecular species through IR spectral profiles of those biomolecules, allowing us to detect and separate biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transport of single-stranded DNA molecules (oligonucleotides) in the micro-fluidic channels.
KW - DNA
KW - Infrared spectroscopy
KW - Micro-fluidic channel
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U2 - 10.1016/j.snb.2016.07.003
DO - 10.1016/j.snb.2016.07.003
M3 - Article
AN - SCOPUS:84979729451
SN - 0925-4005
VL - 238
SP - 917
EP - 922
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -