Labeling of islet cells with iron oxide nanoparticles through DNA hybridization for highly sensitive detection by MRI

Narufumi Kitamura, Ryusuke Nakai, Haruyasu Kohda, Keiko Furuta-Okamoto, Hiroo Iwata

研究成果: ジャーナルへの寄稿学術論文査読

20 被引用数 (Scopus)

抄録

A labeling method for islet cells with superparamagnetic iron oxide nanoparticles (SPIOs) based on DNA hybridization is proposed for monitoring of transplanted islets by magnetic resonance imaging (MRI). The surfaces of SPIOs were modified by via Michael reaction by reacting oligo-(deoxyadenylic acid)-bearing a terminal thiol group at the 5′-end ((dA)20-SH) with maleic acid functional groups on the SPIOs. The SPIOs were immobilized on islet cells which had been pretreated with oligo-(thymidylic acid)-poly(ethylene glycol)-phospholipid conjugates ((dT)20-PEG-DPPE) through DNA hybridization. Transmission electron microscopy observations revealed that SPIOs were initially anchored on the islet cell surfaces and subsequently transferred to endosomes or exfoliated with time. The SPIO-labeled islet cells could be clearly detected as dark spots by T2â̂ - -weighted MR image, whereas non-labeled islet cells could not be detected.

本文言語英語
ページ(範囲)7175-7181
ページ数7
ジャーナルBioorganic and Medicinal Chemistry
21
22
DOI
出版ステータス出版済み - 2013 11月 15

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