Lipid bilayer array for simultaneous recording of ion channel activities

Ayumi Hirano-Iwata; Tomohiro Nasu; Azusa Oshima; Yasuo Kimura; Michio Niwano

doi:10.1063/1.4736263

Lipid bilayer array for simultaneous recording of ion channel activities

Ayumi Hirano-Iwata, Tomohiro Nasu, Azusa Oshima, Yasuo Kimura, Michio Niwano

材料科学高等研究所

研究成果: Review article › 査読

12 被引用数 (Scopus)

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This paper describes an array of stable and reduced-solvent bilayer lipid membranes (BLMs) formed in microfabricated silicon chips. BLMs were first vertically formed simultaneously and then turned 90° in order to realize a horizontal BLM array. Since the present BLMs are mechanically stable and robust, the BLMs survive this relatively tough process. Typically, a ∼60 yield in simultaneous BLM formation over 9 sites was obtained. Parallel recordings of gramicidin channel activities from different BLMs were demonstrated. The present system has great potential as a platform of BLM-based high throughput drug screening for ion channel proteins.

本文言語	English
論文番号	023702
ジャーナル	Applied Physics Letters
巻	101
号	2
DOI	https://doi.org/10.1063/1.4736263
出版ステータス	Published - 2012 7月 9

ASJC Scopus subject areas

物理学および天文学（その他）

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10.1063/1.4736263

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abstract = "This paper describes an array of stable and reduced-solvent bilayer lipid membranes (BLMs) formed in microfabricated silicon chips. BLMs were first vertically formed simultaneously and then turned 90° in order to realize a horizontal BLM array. Since the present BLMs are mechanically stable and robust, the BLMs survive this relatively tough process. Typically, a ∼60 yield in simultaneous BLM formation over 9 sites was obtained. Parallel recordings of gramicidin channel activities from different BLMs were demonstrated. The present system has great potential as a platform of BLM-based high throughput drug screening for ion channel proteins.",

author = "Ayumi Hirano-Iwata and Tomohiro Nasu and Azusa Oshima and Yasuo Kimura and Michio Niwano",

note = "Funding Information: This work was supported by Grant-in-Aids from Japan Society for the Promotion of Science (Grant Nos. 21350038 and 24350032) and JST (PRESTO). Finally, one (A.O.) of the authors thank the Japan Society for the Promotion of Science for research fellowships. ",

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AU - Hirano-Iwata, Ayumi

AU - Nasu, Tomohiro

AU - Oshima, Azusa

AU - Kimura, Yasuo

AU - Niwano, Michio

N1 - Funding Information: This work was supported by Grant-in-Aids from Japan Society for the Promotion of Science (Grant Nos. 21350038 and 24350032) and JST (PRESTO). Finally, one (A.O.) of the authors thank the Japan Society for the Promotion of Science for research fellowships.

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Y1 - 2012/7/9

N2 - This paper describes an array of stable and reduced-solvent bilayer lipid membranes (BLMs) formed in microfabricated silicon chips. BLMs were first vertically formed simultaneously and then turned 90° in order to realize a horizontal BLM array. Since the present BLMs are mechanically stable and robust, the BLMs survive this relatively tough process. Typically, a ∼60 yield in simultaneous BLM formation over 9 sites was obtained. Parallel recordings of gramicidin channel activities from different BLMs were demonstrated. The present system has great potential as a platform of BLM-based high throughput drug screening for ion channel proteins.

AB - This paper describes an array of stable and reduced-solvent bilayer lipid membranes (BLMs) formed in microfabricated silicon chips. BLMs were first vertically formed simultaneously and then turned 90° in order to realize a horizontal BLM array. Since the present BLMs are mechanically stable and robust, the BLMs survive this relatively tough process. Typically, a ∼60 yield in simultaneous BLM formation over 9 sites was obtained. Parallel recordings of gramicidin channel activities from different BLMs were demonstrated. The present system has great potential as a platform of BLM-based high throughput drug screening for ion channel proteins.

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Lipid bilayer array for simultaneous recording of ion channel activities

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