@article{329e8322ea1c4103b427ebfc55426eaa,
title = "LjABCB1, an ATP-binding cassette protein specifically induced in uninfected cells of Lotus japonicus nodules",
abstract = "Legume plants develop root nodules through symbiosis with rhizobia, and fix atmospheric nitrogen in this symbiotic organ. Development of root nodules is regulated by many metabolites including phytohormones. Previously, we reported that auxin is strongly involved in the development of the nodule vascular bundle and lenticel formation on the nodules of Lotus japonicus. Here we show that an ATP-binding cassette (ABC) protein, LjABCB1, which is a homologue of Arabidopsis auxin transporter AtABCB4, is specifically expressed during nodulation of L. japonicus. A reporter gene analysis indicated that the expression of LjABCB1 was restricted to uninfected cells adjacent to infected cells in the nodule, while no expression was observed in shoot apical meristems or root tips, in which most auxin transporter genes are expressed. The auxin transport activity of LjABCB1 was confirmed using a heterologous expression system.",
keywords = "ABC protein, Auxin, Lotus japonicus, Nodule, Symbiotic nitrogen fixation",
author = "Kojiro Takanashi and Akifumi Sugiyama and Shusei Sato and Satoshi Tabata and Kazufumi Yazaki",
note = "Funding Information: We would like to thank the National Bioresource Project (L. japonicus, G. max) for seeds of L. japonicus; Dr. Tsuyoshi Nakagawa (Shimane University, Japan) for pGWB3; Dr. Toshio Aoki (Nihon University, Japan) for the Agrobacterium strain EHA101; Dr. Wolf B. Frommer (Carnegie Institution, USA) for pDR196; Dr. Robert A. Gaxiola (University of Connecticut, USA) for the yeast strain gef1; Dr. Norio Suganuma for seeds of fen1 and sen1 mutants (Aichi University of Education, Japan), and Dr. Shingo Hata (Nagoya University, Japan) for technical advice. DNA sequencing was conducted at the Life Research Support Center at Akita Prefectural University. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 21027022 to K.Y.); Research Fellowships for Young Scientists from the Japan Society for the Promotion of Science (No. 09J00170 to K.T.), and from Mishima Kaiun Memorial Foundation (to K.T.). ",
year = "2012",
month = feb,
day = "15",
doi = "10.1016/j.jplph.2011.11.008",
language = "English",
volume = "169",
pages = "322--326",
journal = "Z. PFLANZENPHYSIOL.",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "3",
}