TY - JOUR
T1 - Mechanism of primary graft non-function in a rat model for fatty liver transplantation
AU - Hatsugai, K.
AU - Ohkohchi, N.
AU - Fukumori, T.
AU - Akamatsu, Y.
AU - Satomi, S.
PY - 2000
Y1 - 2000
N2 - We established a fatty liver model in rat suitable for the model of human liver with steatosis by cholesterol enriched chow, and investigated the mechanism of primary graft non-function in fatty liver transplantation (LTx) using this model. Grafts with steatosis caused primary graft dysfunction after LTx following even short cold preservation; however, no significant difference was recognized in mitochondrial function of the graft during preservation. Morphological findings were not different at 1 h after reperfusion between non-steatotic and steatotic livers. Focal necrosis of hepatocytes was seen and the sinusoidal endothelial cells were injured 24 h after reperfusion. In addition, the fluidity of the plasma membrane decreased in fatty liver. Our results indicate that deterioration of sinusoidal endothelial cells after reperfusion causes graft dysfunction in LTx of steatotic liver.
AB - We established a fatty liver model in rat suitable for the model of human liver with steatosis by cholesterol enriched chow, and investigated the mechanism of primary graft non-function in fatty liver transplantation (LTx) using this model. Grafts with steatosis caused primary graft dysfunction after LTx following even short cold preservation; however, no significant difference was recognized in mitochondrial function of the graft during preservation. Morphological findings were not different at 1 h after reperfusion between non-steatotic and steatotic livers. Focal necrosis of hepatocytes was seen and the sinusoidal endothelial cells were injured 24 h after reperfusion. In addition, the fluidity of the plasma membrane decreased in fatty liver. Our results indicate that deterioration of sinusoidal endothelial cells after reperfusion causes graft dysfunction in LTx of steatotic liver.
KW - Cholesterol-rich chow
KW - Fatty liver transplantation
KW - Mitochondrial function
KW - Plasma membrane
KW - Primary graft non-function
KW - Sinusoidal endothelial cell
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U2 - 10.1111/j.1432-2277.2000.tb02111.x
DO - 10.1111/j.1432-2277.2000.tb02111.x
M3 - Article
C2 - 11112079
AN - SCOPUS:0012531567
SN - 0934-0874
VL - 13
SP - S583-S590
JO - Transplant International
JF - Transplant International
IS - SUPPL. 1
ER -