Monoclonal Antibodies Inhibiting Invasion of Cultured Cells by Eimeria tenella Sporozoites

Tatsuya Uchida, Katsuaki Kikuchi, Hiroshi Takano, Keiji Ogimoto, Yutaka Nakai

    研究成果: Article査読

    6 被引用数 (Scopus)


    Monoclonal antibodies (MoAbs) secreted by 12 hybridomas and reactive with the surface antigens of E. tenella sporozoites were produced. These MoAbs designated as KC-1 to KC-12 were characterized as IgG3K. In Western blot analysis, these MoAbs reacted with only one polypeptide band of sporozoite antigens having a molecular mass of 25 kDa. All the MoAbs were reactive with sporozoites. trophozoites, immature and mature first generation schizonts, first generation merozoites, and the interior structure of sporulating oocysts of E. tenella, however, not with any of the methanol-fixed sporozoites of E. acervulina, E. mitis, E. maxima, E. brunetti, E. necatrix and E. praecox. Invasion of primary culture of chicken kidney cells by E. tenella sporozoites was significantly inhibited by several of the MoAbs. These results suggest that these MoAbs recognizing the E. tenella-specific surface molecule are involved in the invasion of sporozoites into host cells.

    ジャーナルJournal of Veterinary Medical Science
    出版ステータスPublished - 1997 8月

    ASJC Scopus subject areas

    • 獣医学(全般)


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