TY - JOUR
T1 - Novel methylcellulose-immobilized cation-exchange precolumn for on-line enrichment of cationic drugs in plasma
AU - Yamamoto, Eiichi
AU - Sakaguchi, Takahisa
AU - Kajima, Takashi
AU - Mano, Nariyasu
AU - Asakawa, Naoki
PY - 2004/8/5
Y1 - 2004/8/5
N2 - We developed a novel methylcellulose-immobilized strong cation-exchange (MC-SCX) precolumn for direct analysis of drugs in plasma. MC-SCX consists of silica gel with a methylcellulose outer-surface and a 2-(4-sulfophenyl) ethyl phase inner-surface. The MC-SCX precolumn was evaluated by direct analysis using pyridoxine, atenolol and sulpiride spiked in plasma, using a column-switching HPLC system. Each drug was retained and enriched on MC-SCX using an acidic mobile phase, which resulted in good linearity, sufficient reproducibility, intra- and inter day precision, and accuracy in analytical ion-pair LC with trifluoroacetic acid. The analytical methods for model drugs were applied to pharmacokinetics of atenolol and sulpiride in rats.
AB - We developed a novel methylcellulose-immobilized strong cation-exchange (MC-SCX) precolumn for direct analysis of drugs in plasma. MC-SCX consists of silica gel with a methylcellulose outer-surface and a 2-(4-sulfophenyl) ethyl phase inner-surface. The MC-SCX precolumn was evaluated by direct analysis using pyridoxine, atenolol and sulpiride spiked in plasma, using a column-switching HPLC system. Each drug was retained and enriched on MC-SCX using an acidic mobile phase, which resulted in good linearity, sufficient reproducibility, intra- and inter day precision, and accuracy in analytical ion-pair LC with trifluoroacetic acid. The analytical methods for model drugs were applied to pharmacokinetics of atenolol and sulpiride in rats.
KW - Atenolol
KW - Pharmacokinetics
KW - Pyridoxine
KW - Sulpiride
UR - http://www.scopus.com/inward/record.url?scp=2942534599&partnerID=8YFLogxK
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U2 - 10.1016/j.jchromb.2004.04.028
DO - 10.1016/j.jchromb.2004.04.028
M3 - Article
C2 - 15203047
AN - SCOPUS:2942534599
SN - 1570-0232
VL - 807
SP - 327
EP - 334
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -