On the fate of primordial germ cells injected into early mouse embryos

Harry G. Leitch, Daiji Okamura, Gabriela Durcova-Hills, Colin L. Stewart, Richard L. Gardner, Yasuhisa Matsui, Virginia E. Papaioannou

研究成果: ジャーナルへの寄稿総説査読

22 被引用数 (Scopus)

抄録

Primordial germ cells (PGCs) are the founder cells of the germline. Via gametogenesis and fertilisation this lineage generates a new embryo in the next generation. PGCs are also the cell of origin of multilineage teratocarcinomas. In vitro, mouse PGCs can give rise to embryonic germ (EG) cells - pluripotent stem cells that can contribute to primary chimaeras when introduced into pre-implantation embryos. Thus, PGCs can give rise to pluripotent cells in the course of the developmental cycle, during teratocarcinogenesis and by in vitro culture. However, there is no evidence that PGCs can differentiate directly into somatic cell types. Furthermore, it is generally assumed that PGCs do not contribute to chimaeras following injection into the early mouse embryo. However, these data have never been formally published. Here, we present the primary data from the original PGC-injection experiments performed 40 years ago, alongside results from more recent studies in three separate laboratories. These results have informed and influenced current models of the relationship between pluripotency and the germline cycle. Current technologies allow further experiments to confirm and expand upon these findings and allow definitive conclusions as to the developmental potency of PGCs.

本文言語英語
ページ(範囲)155-159
ページ数5
ジャーナルDevelopmental Biology
385
2
DOI
出版ステータス出版済み - 2014 1月 15

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