@article{67b55955964d4126abb5ce56ee2ff7bb,
title = "Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling",
abstract = "EHD3 is localized on the tubular structures of early endosomes, and it regulates their trafficking pathway. However, the regulatory mechanism of EHD3-containing tubular structures remains poorly understood. An in vitro liposome co-sedimentation assay revealed that EHD3 interacted with phosphatidic acid through its helical domain and this interaction induced liposomal tubulations. Additionally, inhibiting phosphatidic acid synthesis with diacylglycerol kinase inhibitor or lysophosphatidic acid acyltransferase inhibitor significantly reduced the number of EHD3-containing tubules and impaired their trafficking from early endosomes. These results suggest that EHD3 and phosphatidic acid cooperatively regulate membrane deformation and trafficking from early endosomes.",
keywords = "EHD, Endosomes, Membrane tubulation, Phophatidic acid, Receptor recycling",
author = "Yuji Henmi and Natsuko Oe and Nozomu Kono and Tomohiko Taguchi and Kohji Takei and Kenji Tanabe",
note = "Funding Information: We thank Abe T. (Okayama Univ.) and Takakuwa Y. (Tokyo Women's Med. Univ.) and his laboratory member for their technical advice. This work was supported by grants from the Ministry of Education, Science, Sports, Culture and Technology of Japan ( 23770148 and 23113721 ) for K. Tanabe and by Research Fellowships of Japan Society for the Promotion of Science for Young Scientists (25.5241) for Y.H. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = mar,
day = "1",
doi = "10.1016/j.yexcr.2016.02.011",
language = "English",
volume = "342",
pages = "1--10",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",
}