TY - JOUR
T1 - Purification and characterization of small and large rubber particles from Hevea brasiliensis
AU - Yamashita, Satoshi
AU - Mizuno, Makie
AU - Hayashi, Hidehiko
AU - Yamaguchi, Haruhiko
AU - Miyagi-Inoue, Yukino
AU - Fushihara, Kazuhisa
AU - Koyama, Tanetoshi
AU - Nakayama, Toru
AU - Takahashi, Seiji
N1 - Publisher Copyright:
© 2017 Japan society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2018
Y1 - 2018
N2 - Natural rubber (NR) is synthesized by the rubber transferase (RTase) on rubber particles (RPs) in latex. Due to the heterogeneity of the RPs in latex, it is difficult to precisely characterize the RTase activity. In this study, we separated the RPs of Hevea brasiliensis with different particle size distributions, via stepwise centrifugations. Analyses of protein compositions and size distributions of NR in the RPs suggest that RPs in Hevea latex can be categorized into two distinct subclasses, the larger RPs (termed 1kRP, 2kRP, and 8kRP) and the smaller RPs (termed 20kRP and 50kRP). Precise enzymatic assays using the RPs revealed that 50kRP showed the highest RTase activity, whereas the larger RPs, which had been regarded to have quite low activity, also exhibited a comparable activity to the smaller RPs. Immunological detections of cis-prenyltransferases in the RPs showed that the abundance of these enzymes correlates with the extent of RTase activity.
AB - Natural rubber (NR) is synthesized by the rubber transferase (RTase) on rubber particles (RPs) in latex. Due to the heterogeneity of the RPs in latex, it is difficult to precisely characterize the RTase activity. In this study, we separated the RPs of Hevea brasiliensis with different particle size distributions, via stepwise centrifugations. Analyses of protein compositions and size distributions of NR in the RPs suggest that RPs in Hevea latex can be categorized into two distinct subclasses, the larger RPs (termed 1kRP, 2kRP, and 8kRP) and the smaller RPs (termed 20kRP and 50kRP). Precise enzymatic assays using the RPs revealed that 50kRP showed the highest RTase activity, whereas the larger RPs, which had been regarded to have quite low activity, also exhibited a comparable activity to the smaller RPs. Immunological detections of cis-prenyltransferases in the RPs showed that the abundance of these enzymes correlates with the extent of RTase activity.
KW - Cis-prenyltransferase
KW - Hevea brasiliensis
KW - Latex
KW - Natural rubber
KW - Rubber particle
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U2 - 10.1080/09168451.2017.1401913
DO - 10.1080/09168451.2017.1401913
M3 - Article
C2 - 29191089
AN - SCOPUS:85049641395
SN - 0916-8451
VL - 82
SP - 1011
EP - 1020
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 6
ER -