TY - JOUR
T1 - Quantitative analysis of 3,4-dimethylmethcathinone in blood and urine by liquid chromatography-tandem mass spectrometry in a fatal case
AU - Usui, Kiyotaka
AU - Aramaki, Tomomi
AU - Hashiyada, Masaki
AU - Hayashizaki, Yoshie
AU - Funayama, Masato
N1 - Funding Information:
We would like to thank Dr. Noriaki Shima, of the Forensic Science Laboratory of the Osaka Prefectural Police Department, for his helpful discussions and advice regarding data analysis. This work was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number Grant-in-Aid for Young Scientists (B) 24790637.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/7
Y1 - 2014/7
N2 - We report here the quantitative analysis of cathinone-type designer drug 3,4-dimethylmethcathinone (3,4-DMMC) in blood and urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a fatal case. Abuse of 3,4-DMMC is widespread and a global issue. However, to date, there have been no reports of 3,4-DMMC-related deaths. We encountered a death in which 3,4-DMMC was thought to play a causative role, and successfully identified this designer drug from biological samples by using LC-MS/MS and QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method. For standard samples, detection of 3,4-DMMC in human blood and urine samples in the calibration range (5-400. ng/mL) was successful with recoveries of 85.9-89.4% (blood) and 95.8-101% (urine), limits of detection of 1.03 (blood) and 1.37. ng/mL (urine) and limits of quantification of 5.00 (blood) and 5.38. ng/mL (urine). The concentrations of 3,4-DMMC in blood (external iliac vein) and urine in the case were 27. mg/L and 7.6. mg/L, respectively. Some metabolites, including 3,4-dimethylcathione (DMC) and β-ketone reduced metabolites (β-OH-DMMC and β-OH-DMC), were detected in both blood and urine.
AB - We report here the quantitative analysis of cathinone-type designer drug 3,4-dimethylmethcathinone (3,4-DMMC) in blood and urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a fatal case. Abuse of 3,4-DMMC is widespread and a global issue. However, to date, there have been no reports of 3,4-DMMC-related deaths. We encountered a death in which 3,4-DMMC was thought to play a causative role, and successfully identified this designer drug from biological samples by using LC-MS/MS and QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method. For standard samples, detection of 3,4-DMMC in human blood and urine samples in the calibration range (5-400. ng/mL) was successful with recoveries of 85.9-89.4% (blood) and 95.8-101% (urine), limits of detection of 1.03 (blood) and 1.37. ng/mL (urine) and limits of quantification of 5.00 (blood) and 5.38. ng/mL (urine). The concentrations of 3,4-DMMC in blood (external iliac vein) and urine in the case were 27. mg/L and 7.6. mg/L, respectively. Some metabolites, including 3,4-dimethylcathione (DMC) and β-ketone reduced metabolites (β-OH-DMMC and β-OH-DMC), were detected in both blood and urine.
KW - 3,4-Dimethylmethcathinone
KW - Designer drugs
KW - Drugs of abuse
KW - LC-MS/MS
KW - QuEChERS
KW - Synthetic cathinones
UR - http://www.scopus.com/inward/record.url?scp=84902119148&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902119148&partnerID=8YFLogxK
U2 - 10.1016/j.legalmed.2014.03.008
DO - 10.1016/j.legalmed.2014.03.008
M3 - Article
C2 - 24780695
AN - SCOPUS:84902119148
SN - 1344-6223
VL - 16
SP - 222
EP - 226
JO - Legal Medicine
JF - Legal Medicine
IS - 4
ER -