Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney

Eikatsu Tsutsumi; Kazuhisa Takeuchi; Takaaki Abe; Nobuyuki Takahashi; Taro Kato; Yoshihiro Taniyama; Yukio Ikeda; Sadayoshi Ito; Keishi Abe

doi:10.1016/S0090-6980(97)00059-2

Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney

Eikatsu Tsutsumi, Kazuhisa Takeuchi, Takaaki Abe, Nobuyuki Takahashi, Taro Kato, Yoshihiro Taniyama, Yukio Ikeda, Sadayoshi Ito, Keishi Abe

研究成果: Article › 査読

7 被引用数 (Scopus)

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We isolated a rat homolog of thromboxane (TX) synthase cDNA (~1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up- regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.

本文言語	English
ページ（範囲）	423-431
ページ数	9
ジャーナル	Prostaglandins
巻	53
号	6
DOI	https://doi.org/10.1016/S0090-6980(97)00059-2
出版ステータス	Published - 1997 6月

ASJC Scopus subject areas

生化学
内分泌学

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10.1016/S0090-6980(97)00059-2

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@article{2313d9df701d471881111f7e2abe2677,

title = "Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney",

abstract = "We isolated a rat homolog of thromboxane (TX) synthase cDNA (~1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up- regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.",

keywords = "Gene expression, Hydronephrosis, Prostaglandin",

author = "Eikatsu Tsutsumi and Kazuhisa Takeuchi and Takaaki Abe and Nobuyuki Takahashi and Taro Kato and Yoshihiro Taniyama and Yukio Ikeda and Sadayoshi Ito and Keishi Abe",

note = "Funding Information: This study was supported in part by Grants-in-Aid (06671124, 07457237, and 08671268) from the Ministry of Education, Science, and Culture, Japan, and a grant from the Foundation for Research of Metabolic Disorder, Japan.",

year = "1997",

month = jun,

doi = "10.1016/S0090-6980(97)00059-2",

language = "English",

volume = "53",

pages = "423--431",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier BV",

number = "6",

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TY - JOUR

T1 - Rat kidney thromboxane synthase

T2 - cDNA cloning and gene expression regulation in hydronephrotic kidney

AU - Tsutsumi, Eikatsu

AU - Takeuchi, Kazuhisa

AU - Abe, Takaaki

AU - Takahashi, Nobuyuki

AU - Kato, Taro

AU - Taniyama, Yoshihiro

AU - Ikeda, Yukio

AU - Ito, Sadayoshi

AU - Abe, Keishi

N1 - Funding Information: This study was supported in part by Grants-in-Aid (06671124, 07457237, and 08671268) from the Ministry of Education, Science, and Culture, Japan, and a grant from the Foundation for Research of Metabolic Disorder, Japan.

PY - 1997/6

Y1 - 1997/6

N2 - We isolated a rat homolog of thromboxane (TX) synthase cDNA (~1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up- regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.

AB - We isolated a rat homolog of thromboxane (TX) synthase cDNA (~1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up- regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.

KW - Gene expression

KW - Hydronephrosis

KW - Prostaglandin

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U2 - 10.1016/S0090-6980(97)00059-2

DO - 10.1016/S0090-6980(97)00059-2

M3 - Article

C2 - 9261862

AN - SCOPUS:0030816229

SN - 0090-6980

VL - 53

SP - 423

EP - 431

JO - Prostaglandins

JF - Prostaglandins

IS - 6

ER -

Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney

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