Recoverable phospholipid polymer hydrogels based on covalently complex as cell-container

We propose novel polymeric hydrogel system as a cell container that can encapsulate cells reversibly. A phospholipid polymer bearing 2-methacryloyloxyethyl phosphorylcholine units and p-vinylphenylboronic acid (PMBV) was synthesized to form a hydrogel with a polymer having multivalent hydroxyl groups. The hydrogel was formed not only in water but also even in the biological medium. After fibroblast cells were added to PMBV solution and mixed with PVA, the cells were immobilized into the hydrogel. After dissociation of the hydrogel, the cells were adhered and proliferated as usual on a conventional cell culture plate. That is, the hydrogel system is useful to maintain the cells as a cell container.