TY - JOUR
T1 - Redox sensor proteins for highly sensitive direct imaging of intracellular redox state
AU - Sugiura, Kazunori
AU - Nagai, Takeharu
AU - Nakano, Masahiro
AU - Ichinose, Hiroshi
AU - Nakabayashi, Takakazu
AU - Ohta, Nobuhiro
AU - Hisabori, Toru
N1 - Funding Information:
This work was supported in part by a research fellowship for young scientist (to K. S.) from JSPS , and CREST from JST (to T. H.), and was supported in part by the Nano-Macro Materials, Devices and System Research Alliance, MEXT, Japan . We thank A. Isu and Y. Aizawa for technical assistance.
Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/2/13
Y1 - 2015/2/13
N2 - Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004)) and Redoxfluor (Yano, T., et al. (2010)), have been developed to investigate the redox state of living cells. However, these sensors are not useful in cells that contain, for example, other colored pigments. We therefore intended to obtain simpler redox sensor proteins, and have developed oxidation-sensitive fluorescent proteins called Oba-Q (oxidation balance sensed quenching) proteins. Our sensor proteins derived from CFP and Sirius can be used to monitor the intracellular redox state as their fluorescence is drastically quenched upon oxidation. These blue-shifted spectra of the Oba-Q proteins enable us to monitor various redox states in conjunction with other sensor proteins.
AB - Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004)) and Redoxfluor (Yano, T., et al. (2010)), have been developed to investigate the redox state of living cells. However, these sensors are not useful in cells that contain, for example, other colored pigments. We therefore intended to obtain simpler redox sensor proteins, and have developed oxidation-sensitive fluorescent proteins called Oba-Q (oxidation balance sensed quenching) proteins. Our sensor proteins derived from CFP and Sirius can be used to monitor the intracellular redox state as their fluorescence is drastically quenched upon oxidation. These blue-shifted spectra of the Oba-Q proteins enable us to monitor various redox states in conjunction with other sensor proteins.
KW - Biosensor
KW - Disulfide
KW - Fluorescence
KW - Oxidation-reduction
KW - Redox regulation
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U2 - 10.1016/j.bbrc.2014.12.095
DO - 10.1016/j.bbrc.2014.12.095
M3 - Article
C2 - 25592971
AN - SCOPUS:84922700559
SN - 0006-291X
VL - 457
SP - 242
EP - 248
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -