Stable lipid bilayers based on micro- and nano-fabrication as a platform for recording ion-channel activities

Ayumi Hirano-Iwata, Azusa Oshima, Hideki Mozumi, Yasuo Kimura, Michio Niwano

研究成果: ジャーナルへの寄稿総説査読

16 被引用数 (Scopus)

抄録

In this review, we will discuss our recent approaches for the formation of mechanically stable bilayer lipid membranes (BLMs) by combining with BLM formation and micro- and nano-fabrication techniques. BLMs were prepared across a microaperture fabricated in silicon (Si) chips or nanoporous alumina films using a minimized amount of organic solvent. Although BLMs spanned over the porous alumina film showed better electrical properties, such as background current noise and current transient, BLMs suspended in a thin Si3N4 septum showed a much superior BLM stability. The BLMs showed tolerance to a high voltage of ±1 V, a membrane lifetime of >40 h, and tolerance to repetitive solution exchanges. Application to a drug screening system has been examined by using the human ether-a-go-go-related gene (hERG) potassium channel as an illustrative example. The potentiality of the present system as a platform of the high-throughput analysis for ion-channel protein is also discussed.

本文言語英語
ページ(範囲)1049-1057
ページ数9
ジャーナルAnalytical Sciences
28
11
DOI
出版ステータス出版済み - 2012

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