The cell death machinery controlled by Bax and Bcl-XL is evolutionary conserved in Ciona intestinalis

N. Takada, H. Yamaguchi, K. Shida, D. Terajima, Y. Satou, A. Kasuya, N. Satoh, M. Satake, H. G. Wang

研究成果: Article査読

14 被引用数 (Scopus)


Bax and Bcl-XL are key regulators of apoptosis in mammals. Here we report the functional characterization of two Bcl-2 homologues, ciBax and ciBcl-XL, in a basal invertebrate-chordate ascidian Ciona intestinalis. CiBax is a Ciona homologue of theBH1-3 pro-apoptotic protein Bax, whereas ciBcl-XL is a Bcl-XL-like anti-apoptotic protein. Molecular modeling analysis showed that ciBax and ciBcl-XL share both sequence and structural similarities to human Bax and Bcl-XL, respectively. Like their human counterparts, ciBax could form a homodimer or oligomers as well as heterodimerize with ciBcl-XL, and overexpression of ciBax caused apoptosis that could be attenuated by ciBcl-XL. Mutagenesis studies showed that the BH3 domain of ciBax is critical for its cell death-inducing function and also for its interaction with ciBcl-XL. In Ciona embryos, ectopic expression of ciBax but not its BH3 deletion mutant resulted in cell dissociation and apoptosis after late gastrula stage of embryonic development. Moreover, not only wild type ciBcl-XL but also a mutant ciBcl-XL(F101V), which is unable to interact with ciBax, could block cell dissociation and developmental deficit in Ciona embryos induced by overexpression of ciBax. Taken together, these findings suggest that functional homologues of both the BH1-3 death effector Bax and the pro-survival Bcl-XL exist in sea squirt Ciona intestinalis, and they control the cell death machinery independent of their heterodimerization.

出版ステータスPublished - 2005 12月

ASJC Scopus subject areas

  • 薬理学
  • 薬科学
  • 臨床生化学
  • 細胞生物学
  • 生化学、医学
  • 癌研究


「The cell death machinery controlled by Bax and Bcl-XL is evolutionary conserved in Ciona intestinalis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。