TY - JOUR
T1 - The effect of SIRT6 on the odontoblastic potential of Human Dental Pulp Cells
AU - Sun, Hua Ling
AU - Wu, Yan Ru
AU - Huang, Cui
AU - Wang, Jia Wei
AU - Fu, Dong Jie
AU - Liu, Yin Chen
N1 - Funding Information:
Supported by the National Natural Science Foundation of China (nos. 81070852 and 81171010 ) and the Fundamental Research Fund for the Central Universities.
PY - 2014/3
Y1 - 2014/3
N2 - Introduction The aim of this study was to investigate whether SIRT6 is expressed in human dental pulp as well as the effect of SIRT6 on proliferation and odontoblastic differentiation of human dental pulp cells (HDPCs). Methods Immunohistochemical and immunocytochemical assays were used to detect the expression of SIRT6 in human dental pulp tissue and HDPCs. To determine the effect of SIRT6 on odontoblast differentiation, HDPCs with loss (HDPCs SIRT6 knockdown) and gain (HDPCs SIRT6 overexpression) of SIRT6 function were developed, and their proliferation ability was examined. Odontogenic differentiation of HDPCs was determined by alkaline phosphatase (ALP) activity, ALP-positive cell staining, alizarin red staining, and von Kossa staining. Mineralization-related genes, including ALP, dentin sialophosphoprotein (DSPP), and dentin matrix acidic phosphoprotein 1, were determined by real-time quantitative polymerase chain reaction. Western blot analysis was performed to detect the expression of DSPP protein. Results SIRT6 was found in the dental pulp tissue and HDPCs. SIRT6 knockdown decreased ALP activity in HDPCs; calcium nodule formation ability; and the expression of mineralization-related genes such as ALP, DSPP, and DMP1, whereas these were increased with the overexpression of SIRT6. Conclusions SIRT6 is expressed in human dental pulp and participates in the odontoblast differentiation of HDPCs.
AB - Introduction The aim of this study was to investigate whether SIRT6 is expressed in human dental pulp as well as the effect of SIRT6 on proliferation and odontoblastic differentiation of human dental pulp cells (HDPCs). Methods Immunohistochemical and immunocytochemical assays were used to detect the expression of SIRT6 in human dental pulp tissue and HDPCs. To determine the effect of SIRT6 on odontoblast differentiation, HDPCs with loss (HDPCs SIRT6 knockdown) and gain (HDPCs SIRT6 overexpression) of SIRT6 function were developed, and their proliferation ability was examined. Odontogenic differentiation of HDPCs was determined by alkaline phosphatase (ALP) activity, ALP-positive cell staining, alizarin red staining, and von Kossa staining. Mineralization-related genes, including ALP, dentin sialophosphoprotein (DSPP), and dentin matrix acidic phosphoprotein 1, were determined by real-time quantitative polymerase chain reaction. Western blot analysis was performed to detect the expression of DSPP protein. Results SIRT6 was found in the dental pulp tissue and HDPCs. SIRT6 knockdown decreased ALP activity in HDPCs; calcium nodule formation ability; and the expression of mineralization-related genes such as ALP, DSPP, and DMP1, whereas these were increased with the overexpression of SIRT6. Conclusions SIRT6 is expressed in human dental pulp and participates in the odontoblast differentiation of HDPCs.
KW - Human dental pulp cells
KW - SIRT6
KW - odontoblastic differentiation
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U2 - 10.1016/j.joen.2013.11.010
DO - 10.1016/j.joen.2013.11.010
M3 - Article
C2 - 24565659
AN - SCOPUS:84894527613
SN - 0099-2399
VL - 40
SP - 393
EP - 398
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 3
ER -