The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage

Miyuki Mayanagi, Megumi Nakamura, Akiko Henmi, Yasuyuki Sasano

研究成果: ジャーナルへの寄稿学術論文査読

抄録

Objective: To determine whether the combination of scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and immunostaining would visualize the mineral and protein distribution in the same histological sections. Methods: Paraffin sections of fixed rat hindlimbs were processed for SEM-EDX and subsequently for immunofluorescence staining. Results: The localization of calcium, phosphorus, and carbon with type II collagen could be visualized in the same region of calcified growth plate cartilage on the same section. Conclusions: The combination of SEM-EDX and immunostaining is effective for visualizing mineral and protein distribution in the same histological sections.

本文言語英語
ページ(範囲)461-464
ページ数4
ジャーナルJournal of Oral Biosciences
64
4
DOI
出版ステータス出版済み - 2022 12月

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